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KMID : 0525720220270020069
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Journal of Chitin and Chitosan
2022 Volume.27 No. 2 p.69 ~ p.77
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UVB Protective Effect of Hydrolysate Derived Hermetia Illucens in Human Keratinocytes
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Park Seo-Yeong
Yoo Si-Won
Lee Seo-Hee
Choi Jong-Min
Song Jin-Woo
Han Eui-Jeong
Ahn Gin-Nae
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Abstract
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In this study, we prepared enzymatic extracts using trypsin and pepsin from Hermetia illucens (THI and PHI) and evaluated their effects on oxidative stress in ultraviolet B (UVB)-stimulated keratinocytes. PHI showed a higher extraction yield than THI, but the protein content of THI was higher. When the antioxidant activity was assessed in vitro, both indicated an effect of 25% or more compared to the positive control (L-ascorbic acid). In addition, both THI and PHI decreased the generation of intracellular reactive oxygen species (ROS) in UVB-stimulated keratinocytes, without cytotoxicity at all concentrations used. We explored the efficacy of THI. Results demonstrated that THI effectively reduced apoptosis and sub-G1 DNA content. In addition, it was found that UVB-stimulated keratinocytes protect skin keratinocytes from UVB by regulating the apoptotic pathway, MAPKs, and NF-kB signaling pathways. In conclusion, these results show that trypsin hydrolysate of H. illucens protects cells from oxidative stress in UVB-irradiated human keratinocytes.
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KEYWORD
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Hermetia illucens, Oxidative stress, Apoptosis, UVB, Human keratinocytes
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